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Try this company, they have all kinds of pulsed lasers if you wanted to build on your own a FLIM system
http://markettechinc.net/Products/Femtosecond_Lasers.aspx
good luck
Dr. Ammasi Periasamy
Professor & Center Director
W.M. Keck Center for Cellular Imaging (KCCI)
Biology, University of Virginia
B005, Physical Life Sciences Building (PLSB)
90, Geldard Drive, Charlottesville, VA 22904
(Campus Mail - P.O. Box 400328)
Voice: 434-243-7602 (Office); 982-4869 (lab)
Fax:434-982-5210; Email:[log in to unmask]
http://www.kcci.virginia.edu/contact/peri.php
************************
12th Annual Workshop on FRET Microscopy, March 11-16, 2013
http://www.kcci.virginia.edu/workshop/workshop2013/index.php
*************************
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Craig Brideau
Sent: Monday, August 27, 2012 10:04 PM
To: [log in to unmask]
Subject: Re: white light laser
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Most of the continuum lasers don't emit shorter than ~450nm, so to get as short as 400 might be troublesome.
Craig
On Mon, Aug 20, 2012 at 3:53 PM, Periasamy, Ammasi (ap3t) < [log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I do have the SP5X, a white light laser system with hybrid detector.
> You can use the white light laser for FLIM, the white light laser is a
> pulsed laser. But you need a detector to couple. You can use the
> Becker & Hickl or PicoQuant FLIM system and can be synchronized with
> the Leica scanning system. The FLIM company people will provide a
> hardware and software to collect the FLIM images with the Leica system.
> The Leica company told me that they are working on a hybrid detector
> which can be used for FLIM too. DO not know when that detector will be
> ready for FLIM imaging in the SP5X system!!
>
> Dr. Ammasi Periasamy
> Professor & Center Director
> W.M. Keck Center for Cellular Imaging (KCCI) Biology, University of
> Virginia B005, Physical Life Sciences Building (PLSB) 90, Geldard
> Drive, Charlottesville, VA 22904 (Campus Mail - P.O. Box 400328)
> Voice: 434-243-7602 (Office); 982-4869 (lab) Fax:434-982-5210;
> Email:[log in to unmask] http://www.kcci.virginia.edu/contact/peri.php
> ************************
> 12th Annual Workshop on FRET Microscopy, March 11-16, 2013
> http://www.kcci.virginia.edu/workshop/workshop2013/index.php
> *************************
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Cameron, Lisa
> Sent: Monday, August 20, 2012 4:29 PM
> To: [log in to unmask]
> Subject: Re: white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> We have a white light laser on our Leica SP5X confocal system which spans
> the range of 470nm-670nm.
> It has worked well for us for regular confocal samples and applications,
> but we have not done FLIM on this system.
> I am not sure of the model - it is supplied by Leica (no choice) - our
> system was purchased in Sept 2008.
> We've had a replacement laser since, that has been working fine for us
> (same model as the original as far as I know).
> The max laser power at 488nm on the WLL is about 1/5th the max laser power
> of our 488 line on our Argon laser at last measurement. Feel free to
> contact me if you have further questions - Lisa
>
>
> ---------------------------------------
> Lisa Cameron, Ph.D.
> Director of Confocal and Light Microscopy Core Dana Farber Cancer
> Institute Boston, MA http://microscopy.dfci.harvard.edu
> 617-582-8824
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Vitaly Boyko
> Sent: Monday, August 20, 2012 3:45 PM
> To: [log in to unmask]
> Subject: [CONFOCALMICROSCOPY] white light laser
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> We are looking into white light laser for confocal system.
>
> Could you, please share your experience with the white light laser
> including its performance in FLIM applications. Is 488 nm line strong
> enough? Is there a white light laser with broad spectral coverage that
> would also include 400-450 nm range?
>
> Many thanks in advance,
>
> Vitaly
> 240-342-6217
>
>
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