***************************************************************
*                                                             *
* Data file format for Olympus LSM GB-200 confocal microscope *
*                                                             *
***************************************************************
 
 
For each image (image stack), there are two files, the data
file (*.trs) and condition data file (*.com)
 
 
Data file: *.trs
 
Header Information Data
 
scan mode @(see below)             int (2B)
Y scan start position              int (2B)
Y scan end position                int (2B)
X record start position            int (2B)
X record end position              int (2B)
X-Z   Y line                       int (2B)
X-Z-Y  Y start section             int (2B)
X-Z-Y  Y end section               int (2B)
X-Z-Y  # of steps                  int (2B)
X-t  Y line                        int (2B)
X-t  duel time                     int (2B)
X-t  # of scans                    int (2B)
X-Y-t  duel time                   int (2B)
X-Y-t  # of scans                  int (2B)
ch1 input                          int (2B) <--|
ch2 input                          int (2B)    |
ch3 input                          int (2B)    |
ch1 output                         int (2B)    |  On: 1
ch2  output                         int (2B)   |  Off: 0
ch3 output                         int (2B) <--+
Rch output                         int (2B) <--+  CH1: 1
Gch output                         int (2B)    |  CH2: 2
Bch output                         int (2B) <--+  CH3: 3
range                              unsigned long (4B)
steps                              unsigned long (4B)
# of slices                        int (2B)
B&W / color                        int (2B)
X pixel size                       float (4B)*
Y pixel size                       float (4B)*
Z step size                        float (4B)*
spare                              char (186B)
-----------------------------------------------------------------
 
* set to 0 if not applicable
 
@ scan mode:
 
scan mode                      data
-------------------------------------------
X-Y                                0
X-Y-Z                              1
X-Z                                2
X-Z-Y                              3
X-t                                4
X-Y-t                              5
V proj                             6
FBS                                7
Camera                             8
 
 
 
 
 
 
Condition data file: *.com
______________________________________________________________
 
confocal/non-confocal                        int (2B)
confocal photomultiplier voltage [ch1]       int (2B)
confocal photomultiplier voltage [ch2]       int (2B)
confocal offset [ch1]                        int (2B)
confocal offset [ch2]                        int (2B)
confocal offset [ch3]                        int (2B)
confocal gain [ch1]                          int (2B)
confocal gain [ch2]                          int (2B)
confocal gain [ch3]                          int (2B)
non-confocal photomultiplier voltage [ch1]   int (2B)
non-confocal photomultiplier voltage [ch2]   int (2B)
non-confocal offset [ch1]                    int (2B)
non-confocal offset [ch2]                    int (2B)
non-confocal offset [ch3]                    int (2B)
non-confocal gain [ch1]                      int (2B)
non-confocal gain [ch2]                      int (2B)
non-confocal gain [ch3]                      int (2B)
photomultiplier signal process mode [ch1]    int (2B)
photomultiplier signal process mode [ch2]    int (2B)
transmitted sensor signal process mode       int (2B)
scan field                                   int (2B)
scan speed                                   int (2B)
objective magnification                      int (2B)
X shift value                                int (2B)
Y shift value                                int (2B)
observation mode                             int (2B)
average mode                                 int (2B)
# of average times                           int (2B)
peak stop value                              int (2B)
scale factor                                 int (2B)
comment                                      char
date
time
 
------------------------------------------------------------------
 
 
 
 
P. C. Cheng and T. H. Lin
Advanced Microscopy and Imaging Laboratory (AMIL)
Department of Electrical and Computer Engineering
State University of New York at Buffalo,
Buffalo, NY 14260 USA
(716) 636-3868 (tel and FAX)
e-mail: [log in to unmask]