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Hi Renato,

I also imaged the same type of specimens - bovine cartilage explants, silver grains in photographic emulsion using reflectance confocal on a Leica TCS 4D confocal microscope. We published a couple of papers using this technique combined with immunostaining for type VI collagen together with biochemical analysis of the various tissue fractions.

It worked brilliantly for assessing localisation.

I can send PDFs of the papers offline if your colleague is interested.

Kind regards,

Jacqui


Jacqueline Ross 
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit 
School of Medical Sciences 
Faculty of Medical & Health Sciences 
The University of Auckland 
Private Bag 92019 
Auckland 1142, NEW ZEALAND 

Tel: 64 9 923 7438 
Fax: 64 9 373 7484 

http://www.fmhs.auckland.ac.nz/sms/biru/


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Renato Mortara
Sent: Monday, 25 November 2013 11:30 p.m.
To: [log in to unmask]
Subject: Silver enhanced autoradiography / dark field under confocal ?

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Dear friends,



A colleague is trying to image mouse brain sections that were radioactively labeled (I guess 35S-Methionine) then, immersed in a photographic emulsion to develop particular sites.



The sections were then counter stained with toluidine or similar (bluish) histological dye. The final result ('bright spots, easily seen under a 20x
objective) can be visualized under dark field microscopy.



I wonder if anyone has been able to image through some kind of reflection mode under confocal microscopy.



Thanks !



Renato Mortara



Dr. Renato Arruda Mortara

Parasitology Division

Escola Paulista de Medicina - UNIFESP

Rua Botucatu,  862 6th floor

04023-062

São Paulo SP Brazil

Phone: 55 11 55798306

www.ecb.epm.br/~ramortara