CONFOCALMICROSCOPY Archives

December 1997

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: DNA dye for living cells
From:
Guy Cox <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 3 Dec 1997 14:50:11 +1000
Content-Type:
text/plain
Parts/Attachments:
text/plain (25 lines) 
>Hi,
>
>Members of my lab want to perform living microscopy of yeast (pombe) cells
>expressing GFP and BFP fusion proteins. We are interested in viewing the
>chromatin and would like a fluorescent dye in another wavelength.
>
>


I'd suggest toto or topro (Molecular Probes) - excited with 647nm
line and well out of the way of GFP.  Some of our users do triple
labelling with them with very good results.

                                                Guy Cox

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