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David,

Bleaching is a complicated process with usually unknown factors to
deal with. If you can "see" the bleaching try fitting a function. If
you are lucky it might be a single exponential decay. More likely
than not it will depend on multiple exponential components and the
spread in the z-PSF at different planes in the sample.

If the % bleaching is less than 20% you can use a simple linear
approximation for each plane and the error shouldn't be too bad.

Send me details, if you like.

Mario


>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Hi fellow confocalists
>
>I have observed that my monitoring beam has been bleaching my flurochrome
>(Cy5).  Is there a known formula that relates the amount of bleaching to
>the number of images acquired (or at least a functional form)? (so that I
>may correct for this "background" bleaching)
>
>any help would be much appreciated!
>
>David Depoil
>PhD student
>Valitutti's Team
>U563 CPTP Inserm
>Toulouse
>France


--
________________________________________________________________________________
Mario M. Moronne, Ph.D.
NanoMed Technologies LLC
President and CTO
ph (510) 528-2400

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