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Dear list,

Does anyone have a method of fixing cells for immunofluroescence studies
which will not cause permeabilization of the plasma membrane.  We have
tried a variety of paraformaldehyde concentrations but still get a high
% of permeabilised cells. 

1% ParaF diluted direct into medium gives best results, but a lot of
cells still get permeabilised.

Thanks in advance

Michelle

Dr Michelle Peckham
School of Biomedical Sciences
Worsley Building
University of Leeds
Leeds
LS2 9JT
UK

telephone: 0113 343 4348
fax:       0113 343 4228 
website:   http://www.cell-motility.com