--- Ian Gibbins <[log in to unmask]> schrieb: > Yes - but you need to
use the Import command under the file menu - I
> think they get converted to 8bit working images when they are imported.

That's right. But be carefull with that: the default setting is that every
single image in the stack gets calibrated from 16 to 8 bit independently. If
you need quantitative measurements you have to define the brightest and dimmest
grayvalue for the entire stack. It can become somewhat anoying to figure out
the right borders, but it works.

Steffen


>
> Hope that helps
>
> IAN
>
>
> Susana Castel wrote:
> >
> > I would like to quantifie fluorescence density in stacks of 16-bit tiff
> > images (i would like to have a kinetic). Do you know if it is possible to
> > get some macrosfor NIH-PC or IMageJ to do it?
> >
> > Susanna Castel
> > Microscopia Confocal
> > SCT-UB
>
> --
> Professor Ian Gibbins
> Anatomy & Histology
> Flinders University of South Australia
> GPO Box 2100, Adelaide, SA 5001
> Australia
>
> Phone:  +61-8-8204 5271
> FAX:    +61-8-8277 0085
> Email:  [log in to unmask]


=====
Steffen Dietzel, Dr. rer. nat.
Institut für Anthropolgie und Humangenetik der
Universität München, Germany
phone: +49/89/2180-6713
fax:   +49/89/2180-6719
e-mail: [log in to unmask]  Web:  http://www.sdietzel.de

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