Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Jessica,
I strongly urge you to follow the calibration route, using a stage
micrometer. It will automatically take into account all the variations in
magnification for each objective as well as tube lengths and a variety of
camera factors (chip size, pixel size, etc.). You can use the stage
micrometer to calibrate any imaging software so that you can add an
appropriate micron bar to your images. This approach is good science and
presentation methodology.
Best regards,
Barbara Foster
Microscopy/Microscopy Education
125 Paridon Street, Suite 102
Springfield, MA 01118
PH: 413-746-6931 FX: 413-746-9311 Web: www.MicroscopyEducation.com
Will you be at M&M in San Antonio? If so, don't forget the Tuesday night
seminar on Fluorescence Calibration/ Also, join the tradition of over
10,000 microscopists: participate in our survey at any time during the
meeting and receive a "sweet thank you". Booth #218
@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@
At 03:18 PM 6/4/03 +0100, Puhalla, Susan wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>If you are using a confocal microscope to acquire images, I think most
>confocal software is calibrated to tell you what your pixel size is (or
>voxel size in x,y, and z) at the specimen. Using the pixel size, you could
>then make a scale bar. For instance, if your pixel size is 150nanometers and
>you make a scale bar that is 100 pixels long, then that scale bar would be
>15,000nanometers long or 15 microns.
>
>Alternatively, there are slides you can purchase which have a microscopic
>ruler, a micrometer, etched on it. You can take an image of the micrometer
>with the same objective you are using to image your cells. Then, measure the
>dimensions of the image to make a scale bar.
>
>-----Original Message-----
>From: Jessica Sample [mailto:[log in to unmask]]
>Sent: 04 June 2003 1:28 PM
>To: [log in to unmask]
>Subject: measuring cell size
>
>
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>I am wanting to measure the size of the cells in my image but have no grid
>on the coverslip. Can anyone give me help on how to get a scale on the
>image.
>Thanks
|
