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Dear confocalists,

I am studying cell spreading on fibronectin-coated coverslips and would
like to know about fluorescent probes that could be used to measure cell area.

Ideally, the probe should faithfully delineate the cellular contour, give
enough signal/noise ratio for easy thresholding and, if possible, be used
after (PFA) fixation.

We also would like to be able to combine it with conventional
immunofluorescence.

Thanks.


F Javier Diez-Guerra, PhD
Profesor Titular
Centro de Biologia Molecular Severo Ochoa
Facultad de Ciencias
Universidad Autonoma
Cantoblanco, 28049 Madrid
SPAIN

phone:  +34 91 4978051
Fax:      +34 91 4978087
e-mail: [log in to unmask]