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Hi,

To acquire DAPI using an Hg lamp, you just need to put your DAPI
fluorescence cube on the LSM position (which is empty). Then you do not use
any cofocal filters.   Use a high average for  image acquisition because
elsewhere you will see very strong horizontal black lines on your image.
The images you will obtain are not of very good quality but is sufficient to
localize  nucleus.

Sincerly yours,

Mathieu Fallet
Centre d'Immunologie de Marseille
INSERM-CNRS
 France
>

> Hi,
>
> Can you tell me how to do this??  We have a Leica TSC-SP.
>
> Thanks much,
>
> Deb Berglund
> Montana State University
>
> One can do DAPI widefield on a confocal by using the Hg lamp to
> excite and collecting the image with the PMT.  This satisfies the
> users we have who want to use DAPI.