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We have done that some years ago: Model MA, Reese JL, Fraizer GC.
Measurement of wheat germ agglutinin binding with a fluorescence
microscope. Cytometry A 75A, 874-881 (2009)

Mike Model


On Mon, Feb 29, 2016 at 7:31 PM, Clarence Dunn <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello,
>
> I'm interested in quantifying the number/density of a particular IL-
> receptor in two different adherent cell populations. Most of what I've
> come across in the liturature uses Flow Cytometry and calibration beads
> or Western Blots using a known quantity of a reference proptein.
> However, I'd prefer to include some imaging data...has anyone on this
> ListServ had success using a microscopy-based techniques to generate
> a quantitative, or even semi-quantitative measure of receptor density?
>
> Thanks very much!
>
> Clarence Dunn, Ph.D., MPH
>