Hi Aget,

In our hands PAGFP and PSCFP2 are by far the best at two photon photoswitching.  We have struggled to get Dendra, KikGR, and mEOS to work well, though given the right conditions (low power, long photoactivation times) perhaps things would work better.  I haven't tried Kaede, but it appears that the chromophore sequence is similar to the others I mentioned.

Jay Unruh
Research Advisor
Stowers Institute for Medical Research

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Sent: Tuesday, July 17, 2012 4:46 AM
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Subject: Re: 2photon conversion of Dendra2

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Thank you so much Manja, the websites are really helpful! I also wrote to the developer of Dendra2 who politely replied that they dont have experience with 2p conversion. Then i wrote to the authors of the paper describing 2p conversion by 810nm but got no reply so far (more than a month). It seems IR laser is not good at doing this job. Maybe i can try Kaede which can also be used to track cells. 

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Institute of Physiology II,University of Tübingen Keplerstraße 15, 72074, Tübingen, Germany
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