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Hi Neil,

We have an OMX-V4 including the beam concentration lens for DLM. We do not use DLM very regularly but the few times that we used it it worked ok. Still, the laser power for STORM could be higher…. It really depends on your experiments.

Cheers,
Oliver





Oliver Biehlmaier, PhD | Head of Imaging Core Facility  | Biozentrum, University of Basel | Klingelbergstrasse 50/70 | CH-4056 Basel
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On 30 Oct 2017, at 15:12, Anthony, Neil <[log in to unmask]<mailto:[log in to unmask]>> wrote:

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Hi Ian, thanks for the input.
We're looking at the v4 with the focusing optic as you mentioned.

Can I please ask which version of SoftWoRx you're running, and whether or not you feel that the increased laser density would help the software?

Also, do you know about the z-res?  I guess might be very different between v3 and v4 of course, but if it's something you've looked into, I'd appreciate your thoughts.

Thanks
Neil


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Ian Dobbie
Sent: Friday, October 27, 2017 2:00 PM
To: [log in to unmask]
Subject: Re: GE DeltaVision OMX DLM

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Hi Neil

Our experience is slightly unique but might be informative.

We have a v3 OMX with the DLM module. In our experience the power density is too low for good STORM imaging. However the newer OMXs have a lens for focusing the power down on a smaller area. I have no experience if this is adequate or not.

The reconstruction software is however pretty ropey and we generally use something like rainSTORM for localisation imaging post acquisition.

Ian

Sent from my iPhone

On 27 Oct 2017, at 18:12, Anthony, Neil <[log in to unmask]<mailto:[log in to unmask]>> wrote:

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Hi all,
I was just wondering if anybody has used the single molecule localization add on, DLM, on the GE DeltaVision OMX?  If so I'd love to hear your thoughts on resolution, usage, and anything else that struck you.
Thanks in advance for your time.

Neil









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