HI,

it is a good idea to stain the fat and protein in milk with Nile blue.  488excitaion will show the fat and 633 will show the protein which should be in solution in standard milk.  In addition you can add DAPI and it will show up any bacteria if you have a 405laser for excitation of DAPI. For fungi if I remember correctly you can use some of the plant dyes to show up any hyphal material.Though some cell wall material autofluoresces and you can probably see hyphae/fungal spores using transmission mode.

REgards

Liz

Liz Nickless

Microscopy and Imaging Centre
Senior Technical Officer
Analytical Development Section
Fonterra Innovations
Palmerston North
New Zealand
06 350 4649 x64527



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Patrizia Vaghi
Sent: Wednesday, 8 September 2010 7:37 p.m.
To: [log in to unmask]
Subject: milk request

Hi all,
i wonder if anyone has experience in milk contamination.
In wich way I can datect bacteria, mycoplasm, fungi, generally microorganism?
Are there commercial kit available?
This is a user's request but I have no experience in this topic

Thanks in advance for tour help

Patrizia Vaghi
Laboratorio di Microscopia Confocale

Centro Grandi Strumenti
Università degli Studi di Pavia
Via Bassi 21, 27100 Pavia
Tel 0382.987548   Fax 0382.422251
e-mail [log in to unmask]

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