At the risk of being attacked by 50 people....there used to be a company
called Biorad - with all the talk of new (and outstanding) technologies by
Leica and Zeiss, I was wondering what happened to them. As has been
noted, these companies each do one-up-man-ship....then one is left for
dead after having invested all that money.......unless you keep spending
more money.....
Paul Robinson
Date sent: Tue, 7 Dec 1999 09:44:49 -0800
Send reply to: Confocal Microscopy List <[log in to unmask]>
From: Guy Cook <[log in to unmask]>
Subject: Re: COMPARISON OF CONFOCAL SYSTEMS
To: [log in to unmask]
Another vote for the Leica SP series. I have used it for over a year as
well, and I believe is the most versatile system on the market. I test a
variety of materials with autofluorescing properties, and I have always been
able to configure the bandpass filters with the probe to maximize detection.
The Leica water immersion objectives are superior to any other WI
objectives I've ever used.
The SP also seems to have a more stable operating system than the NT series.
I don't know if I've just been lucky, but I remember the NT having a few
gremlins running around the software.
Cheers,
-Guy
Guy Cook
President
Bacterin
910 Technology Blvd.
P.O. Box 6743
Bozeman, MT 59715
406-582-8184
Fax 406-586-0396
http://www.bacterin.com
ftp.bacterin.com
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]]On Behalf Of Dr. Nina Allen
> Sent: Tuesday, December 07, 1999 8:37 AM
> To: [log in to unmask]
> Subject: Re: COMPARISON OF CONFOCAL SYSTEMS
>
>
> We too have the Leica SP head and like it very much. It is a
> clever design
> and we have found the ability to carefully test for and select emission
> wavelength to be very useful. Optimizing light gathering is also very
> helpful.
> We have used it for over a year now and are happy with it. Nina Allen
> >Mike
> >There are companies that make their living by making filters
> that let through
> >only certain wavelengths. It is a real art and both Chroma and
> Omega should be
> >complemented for these filters. However the ability to use a slit to
> >build your
> >own bandpass filters is truly a fantastic achievement and also very
> >useful for
> >optimizing performance. The cutoff is better defined than a
> conventional band
> >pass filter. You have the control of building your own filter that has a
> >sharper
> >cut off and better liaght transmitted. The Bandpass filter together with
> >sequential scanning allows one to control the emitted light and
> thus you can
> >optimize the light gathering efficiency of the system. It is
> really a better
> >system.
> >If you understand dichorics and bandpass filters used with flow
> cytometers,
> >Confocal microscopes, microscopes and other laser based equipment your
> >will see
> >the fantastic achievement in design that this Leica SP head
> offers. Being a
> >government employee I can not recommend any commerical
> equipment, but I do
> >appreciate technological performance.
> >Bob Zucker
> >
> >Robert M. Zucker, Ph.D
> >U.S. Environmental Protection Agency
> >National Health and Environmental Effects Research Laboratory
> >2525 NC Highway 54, MD 72
> >Research Triangle Park, North Carolina, 27709
> >tel: 919-541-1585 fax 919-541-4017
> >e-mail: [log in to unmask]
>
>
> @@@@@@@@@@@@@@@@@@@@
> Nina Stromgren Allen
> Professor, Department of Botany;
> Director, Cellular and Molecular Imaging Facility
> Box 7612, Department of Botany
> North Carolina State University
> Raleigh, NC 27695-7612
> Phone: 919-515-8382 (Office), 515-3525 (Lab), 515-2727
> (Department Secretary)
> Fax: 919-515-3436
>
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:[log in to unmask]
WEB: http://www.cyto.purdue.edu
|
