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August 2012

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Subject:
focused laser beam size on stage
From:
Tong Yan <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 23 Aug 2012 21:57:54 +0800
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Dear list,

We have one Leica TCS SP5 MP confocal system and I would like to find out how the size of the laser beam on the stage is controlled?  When we change the pixel size to meet Nyquist criteria, does the laser focal point on the specimen change its size accordingly? Will the focused beam has same size to the pixels? What is the smallest focus size of the laser beam on the stage we can achieve? Does two photon excitation has the same scenarios?
Thank you very much in advance for your kind advice.
Best regards,

TONG Yan (Ms) :: National University of Singapore :: 14 Science Drive 4, Singapore 117543 :: (65) 6516 7202 (DID) :: (65) 6779 2486 (Fax) ::) :: [log in to unmask] (E) :: www.nus.edu.sg (W)Company Registration No: 200604346E

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