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January 2020

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Subject:
Re: Detection of near infrared
From:
Zbigniew Mikulski <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 30 Jan 2020 20:22:31 -0600
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Hi Marcel!

We added a Cy7-like filter cube to our AxioScan Z1 which runs on OrcaFlash 4.0 v2 and Colibri7 LED excitation. This is a great combination for imaging of tissues as you get very little autofluorescence in this region. There is not a ton of reagents available in this range, we are using secondary antibodies conjugated to CF750 from Biotium.

Some juicy tonsil image for you
https://imgur.com/r9Zyl6S

Best, Z.

Zbigniew Mikulski, PhD 
Director, Microscopy Core Facility, Instructor | La Jolla Institute for Immunology | 9420 Athena Circle, La Jolla, CA 92037

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