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Hello,
One of our clients needs to preserve a large amount of fixed leaf samples (leaf disks) expressing YFP and CFP tags. He wants to fix the samples with buffered 4% PFA for a few hours and then store them in the cryoprotective solution (e.g., Watson RE et al., Peptides 1986;7:155-159) at -20C until imaging (within 2-3 weeks after fixation). I wonder whether anybody from the plant researcher community has tried this approach and whether it helped to retain CFP or YFP fluorescence at reasonable levels. Are there any other good options? Thank you.
Alexander
Alexander Jurkevic, PhD
Associate Director
Molecular Cytology Core
University of Missouri
120 Life Sciences Center
1201 E. Rollins St.
Columbia, MO 65211-7310
Phone: 573-882-4895
Fax: 573-884-9676
website http://www.biotech.missouri.edu/mcc/
