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Hello,

I'm labelling rat cardiomyocytes with different antibodies. Sometimes I get
what I think is unspecific staining. The staining consists of longitudinal
"strips" with low fluorescence intensity (Alexa-488 labelled 2nd ab's). This
is typical if I use very low concentrations of primary ab's, and I get
similar results in negative control exp. with no primary ab. Apparently, the
2ary ab is the cause of this, but I cannot explain what it stains. Is it
myosin/actin that I see? Have anyone had the same results previously? Any
help would be appreciated! I can send jpg.-files of the images if the
description wasn't good enough.

Regards,
Fredrik