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Colleagues have a new Olympus FV1000 with autofocus and are having difficulties with it and asked our advice.  Based on images they sent us, one example posted at https://www.flickr.com/photos/mcammer/14273268488/ , I told them that I thought that the problem of horizontal lines was adjustments made by the autofocus during the scan.  (Another possibility we entertained was laser fluctuation and still another would be sudden temperature change by the incubator unit.)

Questions:

1.       Do you think we have diagnosed the problem correctly?

2.       Do other people have this problem?

3.       Is there a software setting in the FV1000 software to tell it to check and set the focus only at the beginning of a scan and at the end of a scan with autofocus polling and adjusting suspended during the scan?


Thank you!!
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Michael Cammer, Microscopy Core & Dustin Lab , Skirball Institute, NYU Langone Medical Center
Cell:  914-309-3270   Lab: 212-263-3208
http://ocs.med.nyu.edu/microscopy & http://www.med.nyu.edu/skirball-lab/dustinlab/

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