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Dear Carol Heckman (Bowling Green State University),
thank you for your reply to my enquiry. I'm sorry to be so late but I was in
vcation. Your suggestion is very helpful and indeed, since we are working
with mammals (rats), I think we can reasonably sustain that Tyrosine
Hydroxylase content is decreased based on the lower immunolabeling. Could
you provide a ref to sustain that protein synthesis can be affected in 30
mins?
Thanks again for your interest in our work
Dott. S. Spiga

----- Original Message -----
From: "Carol Heckman" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Wednesday, August 14, 2002 12:01 AM
Subject: Re: area of cells decreased 30 min after naloxone


> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear Dott.
> the content can certainly decrease, as the shortest known turnover
> time for a protein (at least the shortest I have heard of) is about
> 30 minutes.  A lot depends on what kind of biological system you are
> working with in the lab.
>
> Increasing the synthesis in a eukaryotic cell would only likely take
> place by faster translation, in the time order of 30 minutes.  In
> prokaryotic cells, you can do a whole cell cycle in 30 minutes, so
> there is hardly any doubt that transcription could be increased on
> that time scale.
>
> Carol Heckman
> (Bowling Green State University)
>
>
> >Search the CONFOCAL archive at
> >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >>  does anyone know if protein synthesis can change (decrease or
increase) in
> >less than 30 min??
> >Please help
> >Thanks
> >Dott. S. Spiga
>