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Hi Michael,

You can drop the Zeiss .czi file into Fiji. In the Metadata viewing column check the "Display OME-XML metadata" checkbox.  Metadata with times, etc.  opens up in a separate window.

-Peter






--

Peter T. Toth, PhD

Director, Fluorescence Imaging Core

The University of Illinois at Chicago

835 S. Wolcott Ave (MSB), E-325

Phone: (312) 996-0003

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________________________________
From: Confocal Microscopy List <[log in to unmask]> on behalf of Cammer, Michael <[log in to unmask]>
Sent: Wednesday, August 19, 2020 3:43 PM
To: [log in to unmask] <[log in to unmask]>
Subject: Zeiss 880 timing question

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We have been trying to do FRAP experiments on the Zeiss 880, but we are having difficulties getting accurate times.


Typically, we take 2 to 4 before images, then a bleach, and then the recovery sequence.


The problem is that we do not know how to get accurate times for the sequence after the bleaching.  I used to do this on a Leica SP2 and the times were well reported in the metadata, but I cannot find the times in the Zeiss files.  Any help would be appreciated.


Thank you.


Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory

NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016

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Voice direct only, no text or messages:  1-914-309-3270 and 1-646-501-0567