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September 2008

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Subject:
Gold Standard immersion oil
From:
"SUBSCRIBE CONFOCAL Neil M. Kad" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 3 Sep 2008 11:56:27 -0400
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Hi all,

Just want to know what immersion oils people are using to ensure minimal
background.

I recently tried the Cargille FF, but because I'm using TIRF I saw an
enormous change in the image due to the RI difference compared to regular
immersion oils. So I'd like to stick to the usual 1.52 ish.

I'm working at 488nm for your information

Thanks for your help

Neil Kad
University of Essex

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