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Date: | Wed, 21 Oct 2009 22:26:21 -0400 |
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Dear list,
While preparing some confocal imaging results for publication just
recently, I was informed by one of my PhD committee members that I
should try to present my multi-dimensional microscopy images in such
a way that they are easy to see by people who are color blind. Ie:
you should choose colors for your single monochrome channel images
such that when viewed in a merged/overlay image they convey the same
information as you would see in a standard green/red overlay image
for colocalization, etc. I've tried to read up a bit on this topic on
the web and I even searched the confocal listserv archive, but I
could find no definitive set of rules or guidelines for going about
this. The only discussion I could find about this on the listserv
dates back to 1996 and given that imaging has become even more
prevalent in today's biological sciences since then, I'm wondering if
anyone on here now can direct me to a good source or set of journal
guidelines for publishing color image overlays bearing in mind that
some of the readers will be color blind . Also, does anyone know of
any image processing utilities or plugins (ImageJ?) that can covert a
full color image into a version that is easier to interpret by
someone who is color blind?
I've come to realize this is even more important than I previously
thought given that it seems that almost 1 in 10 males worldwide is
color blind (the occurrence of color blindness is said to be lower in
females). Actually, if you want to learn more about the topic of how
humans perceive color and the history of making colors, I highly
recommend a BBC documentary I came across just yesterday called
"Cracking the Colour Code". Very entertaining.
John Oreopoulos
PS: I used both spellings of the word color in the subject line so
that others can find this thread easily in the future.
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