Hi Shea,
If you just want to watch fungal growth on plant (leaf) surface, there are two
ways using regular microscopy with DIC optics. First, you can use leaf vien
(Heath et al., 1997 New Phytol. 135:689-700); or if you are interested in the
leaf surface of stomatal area you may like to try the method discribed by Xu
and Mendgen (1991 Can. J. Bot. 69: 2279-85) in living condition.

Haixin Xu (Ph.D.)
Botany and Plant Pathology
Michigan State University
East Lansing, MI 48824

> > Hello everyone;
>    I have just started looking at a GFP-transformed fungus growing on =
> plant surfaces, using regular fluorescence microscopy.  I can clearly see =
> the fungus using two filter sets: a UV set (ex/em 365/>420) and a blue =
> filter set (ex/em 470/>515).  While the fluorescence in blue light is much =
> brighter, stuff that is blue/blue-white with UV illumination is green in =
> the blue light.  is the "extra" green in the blue light likely to be where =
> the GFP is not as concentrated, and hence doesn't show up in the UV, or is =
> it more likely to be some other compound that has similar excitation/emissi=
> on characteristics?  I have done a fair bit of reading on the subject, but =
> am still feeling a bit vague, and would really appreciate hearing from =
> those of you who have some first hand experience with GFP.
>
> thanks in advance
> shea
>
>
>
> Dr. S.Shea Miller
> Agriculture & AgriFood Canada
> Eastern Cereal and Oilseed Research Centre
> Rm. 2068 Neatby Building
> Central Experimental Farm
> Ottawa, Ontario
> Canada   K1A 0C6
> Phone:  (613) 759-1760
> Fax:  (613) 759-1701
> E-mail:  [log in to unmask]
>