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On further thought. I think your understanding of the term multiplex is 
more correct. The term itself confers a multiplicative aspect as 
demonstrated in the DAPI example given earlier. The addition of more 
colors without further measurements would be additive, not 
multiplicative. Multiplex data generation comes from the application of 
cell morphological identification and measurement algorithms that can 
be applied to both automated and manual imaging.

Cruz
On May 18, 2006, at 8:45 AM, Guy Cox wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Well, all of this contradicts my understanding of the
> term.  As I see it multiplexing is encoding two or more
> data streams within ONE channel so looking at multiple
> channels each containing one data stream can never be
> multiplexing.  Multiplexing may well become involved in
> such applications as remote operation of a microscope.
>
>                                                 Guy
>
>
>
>> My two cents worth.
>> Multicolor measurements are a sub-set of multiplex meausurements.
>> Multiplex refers to several different meausurements made within a 
>> single
>> sample (cell image) this can include color, cell morphology 
>> measurements,
>> etc. For example, a nuclear stain (DAPI) alone can be made multiplex 
>> by
>> the analysis of
>> nuclear shape, size, signal intensity, signal variation, etc.
>>
>> Cruz Hinojos, Ph.D.
>> Baylor College of Medicine
>>
>> ________________________________
>>
>> From: Confocal Microscopy List on behalf of Robert J. Palmer Jr.
>> Sent: Thu 5/18/2006 7:52 AM
>> To: [log in to unmask]
>> Subject: multiplex
>>
>>
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> In my lab we are having a friendly discussion on the derivation and
>> use of the term "multiplex".  What does this term mean and under what
>> conditions is its use appropriate?  For example, does the use of
>> FITC/TRITC/Cy5 qualify as a "multiplex" application?  If so, I would
>> counter that this used to be called, appropriately, "multicolor" so I
>> am not sure why replacement of that previously valid descriptor with
>> the vague (?) term "multiplex" is desirable.  How about the use of
>> several flavors of quantum dots?  This application seems to garner
>> the modifier "multiplex" on a frequent basis.  When and how is this
>> different than "multicolor"?
>> Thanks for the scientific lingo lesson :) :)!
>> Rob Palmer
>> --
>> Robert J. Palmer Jr., Ph.D.
>> Natl Inst Dental Craniofacial Res - Natl Insts Health
>> Oral Infection and Immunity Branch
>> Bldg 30, Room 310
>> 30 Convent Drive
>> Bethesda MD 20892
>> ph 301-594-0025
>> fax 301-402-0396
>>
>>
>>
>
>
> -- 
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
>
> Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
> http://www.guycox.net