Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

As a side note, there are also solid state lasers available with a 490nm
line (Crystalaser), a 491nm line (Cobolt - up to 100mW!) and even 488 nm
(Point Source).

As for custom filter sets, AHF Analysentechnik did a good job for us
(www.ahf.de). In my experience, major players like Chroma don't even bother
to respond to custom filter requests - and I have been told of similar
experiences. I am just saying because it might save you some time.

Best regards,

Kevin

Kevin Braeckmans, Ph.D.
Lab. General Biochemistry & Physical Pharmacy
Ghent University
Harelbekestraat 72
9000 Ghent
Belgium
Tel: +32 (0)9 264.80.78
Fax: +32 (0)9 264.81.89
E-mail: [log in to unmask]
 

> -----Oorspronkelijk bericht-----
> Van: Confocal Microscopy List 
> [mailto:[log in to unmask]] Namens Guy Cox
> Verzonden: dinsdag 13 maart 2007 5:15
> Aan: [log in to unmask]
> Onderwerp: Re: lasers for LSM
> 
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> The efficiency of exciting fluorochromes is only part of it.  
> 473 will be less good than 488 at exciting FITC but the 
> window for detecting FITC will be much wider with the 
> combination Maria proposes, so in the long run she'll 
> probably do better.
> That does depend on a custom triple dichroic being available 
> for the 473, 559 and 635 combination.
> Without that there will be a problem ...
> 
>                                            Guy
> 
> 
> 
> > Hi Maria,
> > If you go to either of these sites, you can see where common laser 
> > lines intersect the the spectra of fluorescent dyes and 
> proteins, and 
> > hence their efficiency.  This, of course, does not take 
> into account 
> > the power output of the lasers.
> >
> > http://www.mcb.arizona.edu/IPC/spectra_page.htm
> >
> > http://www.mcb.arizona.edu/ipc/fret/
> > Cheers,
> > Carl
> >
> > Carl A. Boswell, Ph.D.
> > Molecular and Cellular Biology
> > University of Arizona
> > 520-954-7053
> > FAX 520-621-3709
> > ----- Original Message -----
> > From: "Maria Jimena Ortega" <[log in to unmask]>
> > To: <[log in to unmask]>
> > Sent: Monday, March 12, 2007 2:30 PM
> > Subject: lasers for LSM
> >
> >
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> >
> > Hi,
> > we use a system with 488, 543 and 633 lasers for common 
> fluorochromes.
> > We are thinking of changing to a system with the 473, 559 and 635 
> > diodes lines.
> > Does anyone have a comparison of the eficiency of this 
> lines for most 
> > used fluorochromes?
> >
> > Thanks a lot
> >
> > Maria
> >
> 
> 
> --
> Associate Professor Guy Cox
> Electron Microscope Unit,
> University of Sydney,
> NSW 2006, Australia
> 
> Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
> http://www.guycox.net
>