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Yes,
Nikon (200 mm tube lens) 16x/0.8 has to have BFP diameter of 20 mm. So with 
small Olympus thread you won't be able to use the whole NA of your big Nikon
lens...

You may also need to rise the stage if the parfocal distance of the Nikon 
lens is much larger than that of the Olympus lenses (i.e. the Nikon lens is 
much longer). But if you manage to fit it mechanically, it should work.

Best, zdenek



---------- Původní zpráva ----------
Od: Craig Brideau <[log in to unmask]>
Komu: [log in to unmask]
Datum: 26. 11. 2014 14:08:33
Předmět: Re: inter-manufacturer objective compatibility

"*****
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Actually the larger, newer Olympus lenses have switched to M25 thread as
well. In this case it depends on what your nosepiece is threaded for. RMS
is fairly small and limits the back aperture of objectives pretty
drastically, so the move has been towards M25, and even M32 on big bore
objectives like the 16X/0.8 and 25X/1.1 sold by Nikon. The back aperture of
these lenses is on the order of 18mm or so!

Craig

On Wed, Nov 26, 2014 at 11:47 AM, Jens-Bernhard Bosse <[log in to unmask]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Maurice,
>
> Olympus objectives have a RMS thread, while Nikon uses M25. You can get
> adapters at Thorlabs (no commercial interest).
> Also the parfocal distance is different which might result in a problem
> with your z-drive.
> This is a good summary:
> http://www.microscopyu.com/articles/optics/cfintro.html (no commercial
> interest).
> Not sure what you mean with beam expander. Maybe you mean tube lens?
> The tube lens focal length for Nikon is 200 mm while Olympus uses 180 mm.
> But that should not be a big problem.
> Both companies do their optical corrections in the objective and not the
> tube lens, I believe. So mixing Leica and Zeiss with the "Japanese"
> manufacturers might be a bigger problem. Maybe someone on the list can
> confirm?
>
> Hope that helps,
>
> Jens
>
>
> > On Nov 26, 2014, at 1:23 PM, Needham, Maurice <[log in to unmask]>
> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Everyone,
> >
> > We have an Olympus two-photon system that we would like to use a Nikon
> 16X lens (CFI LWD 16XW, NA 0.8, WD 3mm) with. We realize that the beam
> expander on the Olympus will not compensate for this lens but wondered if
> anyone has experience with “fooling” the software to allow compensation
> when using non-Olympus lenses. Also, we would appreciate comments on the
> utility of this lens for two-photon imaging as we also have a custom 
system
> that won’t have the beam expander problem.
> >
> > In addition, does anyone know of a supplier or accurate method of
> fabricating adaptors to allow use of objective lens between different
> manufacturer's scopes. Different objective screw thread is the main issue.
> >
> > Thanks for your help,
> >
> > Maurice
>"