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Hi,
I'm trying to diagnose an odd problem with an FV-1000 and IX-81  
microscope, to get speed things in getting this serviced.  When  
collecting a z-series, around 330 to 400 µm into the volume, the  
image shifts in the x-axis by several pixels to the left, sometimes 1  
or 2 lines upwards.  The shift may occur within 1 line, or up to 15  
lines resembling a vibration, but trending to the left.  in the  
subsequent portion of the stack, additional shifts will occur every  
40 to 120 µm.  If I capture the full volume in increments of less  
than 400 µm, the shift will still occur.  I've used box sizes from  
256 to 1024 at dwell times of 2 µs to 20 µs.  At 2 µs with a 256x256  
box, the shift doesn't occur within a frame, but seems to be between  
the frames.  Also, the shift always occurs in the upper 1/3 of an image.

In line sequential acquisition, the shift appears in all channels.   
With sequential frame acquisition, the shift appears within a frame  
of the first channel but the slice images for the other channels  
shift in their entirety.

The air table is up, although vibrations shouldn't be this regular.   
Also, the starting point of the z-series may vary, relative to the  
coverslip.

Any suggestions?

Regards,
Glen


Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
(206) 616-4156
[log in to unmask]

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The box said "Requires WindowsXP or better", so I bought a Macintosh.
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