*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****
FYI recent publication of lightsheet microscopy of tumor spheroids.
http://www.researchgate.net/publication/51862390_Live_cell_division_dynamics_monitoring_in_3D_large_spheroid_tumor_models_using_light_sheet_microscopy
https://www.youtube.com/watch?v=72OPWhC-zy4
Cheers, jens
On Thu, Apr 10, 2014 at 6:40 PM, Peter Gabriel Pitrone
<[log in to unmask]>wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello Doug,
>
> I agree with Diaspro (even though he didn't come out and directly say
> it), Lightsheet microscopy would be better in this particular experiment.
> I'll make a shameless attempt at self promotion as well, check out
> OpenSPIM: http://www.openspim.org ;-)
>
> Best Regards,
> Pete
>
> --
> Peter Gabriel Pitrone - FRMS TechRMS
> Microscopy/Imaging Specialist
> Prof. Dr. Pavel Tomancak group
> Max Planck Institute for
> Molecular Biology and Genetics
> Pfotenhauerstr. 108
> 01307 Dresden
>
> http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html &
> http://www.openspim.org
>
> "If a straight line fit is required, obtain only two data points." - Anon.
>
>
> On Thu, April 10, 2014 17:43, Alberto Diaspro wrote:
> <|> *****
> <|> To join, leave or search the confocal microscopy listserv, go to:
> <|> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> <|> Post images on http://www.imgur.com and include the link in your
> posting.
> <|> *****
> <|>
> <|> We got some super resolution on spheroids
> <|> http://www.nature.com/nmeth/journal/v8/n12/full/nmeth.1744.html
> <|>
> <|> alby
> <|>
> <|> www.nic.iit.it
> <|>
> <|>
> <|> Il giorno 10 apr, 2014, alle ore 17:38, Cromey, Douglas W - (dcromey)
> <|> <[log in to unmask]> ha scritto:
> <|>
> <|>> *****
> <|>> To join, leave or search the confocal microscopy listserv, go to:
> <|>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> <|>> Post images on http://www.imgur.com and include the link in your
> <|>> posting.
> <|>> *****
> <|>>
> <|>> I am working with a lab that is interested in doing fluorescence
> <|>> microscopy on spheroid assays (clumps of cancer cells seeded and
> <|>> growing inside a moderately thick collagen matrix). They are looking
> <|>> at a number of different microscopy techniques on campus. Because our
> <|>> Leica confocal was mostly configured for 2D cultured cell and tissue
> <|>> sections, we have quickly discovered the working distance limitations
> <|>> of our existing objectives. Our local Leica technical representative
> <|>> will be loaning us Leica's fabulous 25x/0.95 water immersion objective
> <|>> (2.5mm WD) to try out. This should help with WD issues, as well as
> <|>> spherical aberration in the sample (their dishes do at least have #1.5
> <|>> thickness glass coverslip bottoms).
> <|>>
> <|>> Has anyone else worked with these types of assays before? Any
> <|>> suggestions on the sample prep side or the imaging side to end up with
> <|>> better image data?
> <|>>
> <|>> Thanks,
> <|>> Doug
> <|>>
> <|>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> <|>> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> <|>> Dept. of Cellular & Molecular Medicine, University of Arizona
> <|>> 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA
> <|>>
> <|>> office: AHSC 4212 email: [log in to unmask]
> <|>> voice: 520-626-2824 fax: 520-626-2097
> <|>>
> <|>> http://swehsc.pharmacy.arizona.edu/micro
> <|>> Home of: "Microscopy and Imaging Resources on the WWW"
> <|>>
> <|>> UA Microscopy Alliance -
> <|>> http://microscopy.arizona.edu<http://microscopy.arizona.edu/>
> <|>
>
|
