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Dear Scott and the List,
I bet it's nailpolish---somebody got a little
anxious and didn't wait for their seals to dry. Still
there usually isn't all that much on a slide, so I
imagine "covered" might be a little strong.
At any rate, nail polish remover is xylene. I
think with Zeiss this wouldn't be too horrible a
problem, but am not sure about Nikon. So I would call
your Nikon rep and find out just how sturdy the
chemical coatings on your lense are.
But I am about to wreck havoc here with imaging
heresy and will probably be barred forever from using
common equipment..... BUT. Personally, I do ALL of
the things they tell you NOT to do. I wipe goop off
with Kimwipes, I use chloroform to clean immersion oil
off of inverted 100X objectives, and I have been known
to drip acetone on objectives as well (I microinject
and use the acetone to clean needles). And even
though I have put some valiant effort into the
ridiculous purple sparkle stuff they tell you is the
miracle cleaner, I have never found it up to the task
of removing type B immersion oil. (I only do
heretical things on MY equipment--never on shared
equipment. Randy).
But you know what? None of the horrible things they
warn you about has ever happened to me! I have never
had a lense cloud up, unuseable because I cleaned it
with solvents. I have never had a lense become
scratched because I let it come in contact with cotton
fibers or for any other reason, really. I routinely
clean oil immersion lenses under a dissecting scope
with ethanol and other solvents, and really, I am
completely sanguine.
So if it were me? I'd go to Walgreens and buy some
nail polish remover, and very gently clean the lens
under a dissecting scope, with kimwipes and lense
paper and cotton-tipped applicators, after I called
Nikon first of course. But it wouldn't surprise me to
hear them say, "Oh god, you're going to have to buy a
brand new lens! Ka-ching" in which case you'd have
nothing to lose anyway......
Alice Schmid
--- Scott Snyder <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Are you sure it is nail polish and not some
> combination of incompatible
> immersion oils?
>
> > ----------
> > From: Ricky Critcher
> > Reply To: Confocal Microscopy List
> > Sent: Thursday, October 3, 2002 5:46 AM
> > To: [log in to unmask]
> > Subject: nail polish
> >
> > Search the CONFOCAL archive at
> >
>
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hi
> >
> > A bit off topic I know, but I have just discovered
> an objective (Nikon
> > 60X,
> > Plan Apo) covered in nail polish.
> > Any ideas on the best way to clean this off?
> >
> > Thanks for your help
> >
> > Rick
> >
> > R. Critcher
> > Dept. of Genetics
> > Cambridge Uni
> > UK
> >
> >
>
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