Hi, 
Does anybody no of anyone who is looking for a very nice confocial? we have
lost funding and must get rid of our Zeiss LSM 510NLO ready. We must find a
home for this soon
Thanks



On Thu, 22 Oct 2009 16:40:17 -0400, Mike Tighe
<[log in to unmask]>
wrote:
> If total volume is 550mls (500ml media and 50mls serum you will have 
9.1% 
> serum. Not 10% but probably enough to keep most cells happy
> 
> Mike
>  
>>>> "B. Prabhakar Pandian" <[log in to unmask]> 10/22/09 3:10 PM >>> 
> Hello,
>              In our laboratory there has been recent debates on the cell 
> media preparation. The point of contention is the presence of serum in 
> media.
> When a media mentions supplemented with 10% serum, which of the 
> following is correct for a 500ml media bottle.
> 
> 1. Add 50ml of serum to the media bottle (total volume is 550ml)
> 
> 2. Remove 50ml of media and then add 50ml of serum (total volume is
500ml).
> 
> Any information on the right usage will be great.
> 
> Thanks,
> 
> - Bala