I have "played with" the Leica resonant scanner and I was quite impressed. The imaging is very fast and the images are relatively resolved given the speed. The system is also very easy to use and allows you to average and to utilize an 8kx8K resolution which cleans up the image considerably. However, once you perform considerable averaging and use such a high resolution you lose the speed. The take home message is that you have a lot of flexibility to perform either; very fast somewhat noisy imaging, or fast and somewhat resolved imaging, and everywhere in between. What settings you choose depends entirely on what you want to accomplish.
Cheers,
Brian D Armstrong PhD
Light Microscopy Core Manager
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: Friday, February 06, 2009 4:13 AM
To: [log in to unmask]
Subject: Re: Input on the Leica STED
Well, maybe Moji will sue - I believe he registered a trademark!
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of simon walker (BI)
Sent: Friday, 6 February 2009 11:08 PM
To: [log in to unmask]
Subject: Re: Input on the Leica STED
They're obviously using the term in a different context now.
From the Leica website:
High Speed and High Resolution - All in One The Leica Tandem Scanner combines two technological solutions in one system: a conventional scanning system - ideal for morphological imaging and three dimensional structure analyses, and a resonant scanning system - the best solution for high speed confocal imaging.
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: 06 February 2009 12:04
To: [log in to unmask]
Subject: Re: Input on the Leica STED
Well, tandem scanning was the term coined by Mojimir Petran for his spinning-disk confocal system. Ever since then there have been companies offering similar systems. Leica used to offer such a system (based on the BD CARV) but have since discontinued it. But it is something very different in all respects (including both price and performance) from the Leica STED which is a super- resolution system offering 85nm resolution.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of simon walker (BI)
Sent: Friday, 6 February 2009 10:46 PM
To: [log in to unmask]
Subject: Re: Input on the Leica STED
Presumably we're talking about the SP5 with conventional and resonant scanners and adapted for STED..?
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Guy Cox
Sent: 06 February 2009 11:41
To: [log in to unmask]
Subject: Re: Input on the Leica STED
Hello, what are we talking about here? I've used the Leica STED (and I'm seriously impressed with it) but I didn't know Leica made a tandem scanner. Please clarify! I'm presenting results from the Leica STED at NZCM next week in Rotorua and in April at FOM in Krakow. I do realise both are a bit remote from Brazil.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________
Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Renato Mortara
Sent: Friday, 6 February 2009 9:37 PM
To: [log in to unmask]
Subject: Input on the Leica STED
Hi,
I would appreciate any input on the Leica Tandem Scanner confocal microscope (pros and cons).
Many thanks !
Renato
Renato A. Mortara
Parasitology Division
UNIFESP - Escola Paulista de Medicina
Rua Botucatu, 862, 6th floor
São Paulo, SP
04023-062
Brazil
Phone: 55 11 5579-8306
Fax: 55 11 5571-1095
email: [log in to unmask]
home page: www.ecb.epm.br/~ramortara
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