Dear Martin,

we are currently hosting/testing the Fluoview FV10i (oil variant) in our facility.
I really like the concept and the hardware of the instrument. Unfortunately I have to say that I'm not very convinced of the software that comes with the system. Just to give you an example I really don't understand why Nyquist sampling is considered to be optimal (Button for optimal imaging conditions) in the z-direction but not in the xy-plane. Especially in a facility I find this more confusing than helpful. I also have to impression that there are artificial software brakes so that the potential of the hardware cannot be fully used.

The oil variant cannot be heated thus it is not suitable for live cell imaging.

Just my two pence.

Cheers Arne   

---------------------------------------------------------------
Dr. Arne Seitz
Head of Bioimaging and Optics Platform (BIOP)
Swiss Institute of Technology (EPFL) 
Faculty of Life Sciences 
Station 15 
CH-1015 Lausanne 

Phone: +41 21 693 9618
Fax:      +41 21 693 9585
http://biop.epfl.ch/
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Martin Wessendorf
Sent: lundi, 5. octobre 2009 22:11
To: [log in to unmask]
Subject: Olympus FE10i

Has anyone had any experience with the Olympus FE10i 
"confocal-in-a-box"?  Seems as if it could be useful for live-cell 
imaging in a core facility but I'd be interested to know whether or not 
it lives up to its potential.

Thanks--

Martin Wessendorf
-- 
Martin Wessendorf, Ph.D.                   office: (612) 626-0145
Assoc Prof, Dept Neuroscience                 lab: (612) 624-2991
University of Minnesota             Preferred FAX: (612) 624-8118
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: (612) 626-5009
Minneapolis, MN  55455                    e-mail: [log in to unmask]