CONFOCALMICROSCOPY Archives

February 1996

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: theorical resolution
From:
Lothar Blatter <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 9 Feb 1996 09:12:00 CST
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Dear Guy Cox (and to others who have measured this):
   what is your FWHM in the x, y, and particularly z-dimension using
100 nm beads and the mentioned objective (63x, 1.4 N.A.). I recently
measured this on our Zeiss LSM410 and was disappointed: 1.8 um FWHM
with a Zeiss Plan-Neofluar (40X N.A. 1.3 oil) in the z-axis. In the y
x dimension the FWHM was as expected diffraction limited.
Thans for your imput
Lothar A. Blatter
Dept. Physiology, Loyola University Chicago
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