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November 2012

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Subject:
Tissue prep for TIRF
From:
Adele Tufford <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Sun, 18 Nov 2012 13:25:15 -0600
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Hello, 

I need to image fixed cryosections of brain tissue on an Olympus TIRF 
microscope. 
My guess is that I can't use conventional specimen slides (ie Fisher Superfrost 
+) to collect tissue. Has anyone had any experience collecting tissue on 
Coverslips, and preparing them for TIRF. 

Thank you!

Adele Tufford, McGill University

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