CONFOCALMICROSCOPY Archives

March 1997

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Subject:
Re: fluorescent dyes for plant seed coats
From:
Tobias Baskin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 6 Mar 1997 12:25:32 -0600
Content-Type:
text/plain
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text/plain (18 lines) 
Greetings,
        My bet is that many plant sees coats are massively autofluorescent.
This will be a boon if your goal is simple observation, but a boon if you
want to stain specific structures. You can check by just mounting a hunk of
the seed coat in water and putting it under the microscope. Fixation can
make autofluorescence worse, but I don't think it can make it less.
        Just my few sou.



      _      ____   ^      __   ____   Tobias I. Baskin
     /  \   /      / \    /   \ \            University ofMissouri
    /   |  /      /   \   \      \              BiologicalSciences
   /___/  /__    /___  \   \      \__            109 Tucker Hall
  /      /      /       \   \      \            Columbia, MO 65211 USA
 /      /      /         \   \      \          voice: 573-882-0173
/      /____  /           \   \__/   \____ fax: 573-882-0123

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