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August 2021

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Subject:
Re: RNAscope HiPlex detection
From:
Ted Usdin <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 25 Aug 2021 12:04:11 -0500
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Thank you for the suggestions.

It seems that at 750nm an endogenous substance is excited, and swamps out the Alexa 750 signal. Reducing the excitation wavelength allows the specific signal to be seen.

(And yes, GD, I will try tau)

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