Dear Ian, Bio-Rad Argon laser is 25mWatts. We have many users doing
     FRAP work. If you tell me exactly what you want to do, and with which
     fluorochromes, maybe I can help.


     Anna Smallcombe PhD     Applications (Bio-Rad UK)


______________________________ Reply Separator _________________________________
Subject: FRAP & Lasers
Author:  Confocal Microscopy List <[log in to unmask]>  at
Internet
Date:    04/12/97 09:10


Question on behalf of a colleague intending confocal purchase:

Does anyone have experience with or can comment on appropriate lasers
for FRAP work.  A colleague is trying to make a budgetary decision,
and has a choice between

new 75mW ArKr (triple line),
and

new 15mW Ar (488, 514nm) + their own red HeNe.

BioRad have suggested the 15mW laser is more stable and will supply
enough  power for photobleaching. This sounds correct, but we do need
actual confirmation from users if possible. Comments from any users
with similar setups would be appreciated.

Thanks

Ian.




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Dr Ian S. Harper
Confocal Microscopy Facility
Department of Biological Sciences
Monash University, Clayton, Vic 3168, Australia
Email: [log in to unmask]
Tel: +61 3 9905 5635;  Fax: +61 3 9905 5613
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