Please note the following meeting that may be of interest to some on this=
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listserve.
 
WORKSHOP
 
PCR-IN SITU HYBRIDIZATION: =20
What you need to know to make it work
 
As part of the Histochemical Society Annual Meeting,=20
August  2-3, 1996,=20
Place: Hyatt Regency Hotel, Bethesda, MD
 
=09Topic: The use of PCR has revolutionized study of low-abundance=20
DNA and RNA molecules and recent advances have made it possible to=20
amplify DNA and cDNA targets in fixed cells and paraffin-embedded tissues=
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on slides.  The use of these techniques is beginning to have a major=20
impact in both  cell biology  and diagnostic pathology.  The workshop=20
will deal with: =20
=09=A5methodologies to label and detect nucleic acids using=20
non-radioactive primers=20
=09=A5advantages and disadvantages of PCR-ISH, pitfalls, controls,=20
optimal protocols, effects of fixatives, protease digestion, primers,=20
nucleotides, relevant enzymes and related issues. =20
=09=A5planned format includes lectures, question periods and=20
interactive  demonstrations of  in situ-PCR,  PCR-in situ hybridization=20
and reverse transcriptase-in situ PCR.  Thermocycling systems appropriate=
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for processing selected tissue specimens will be utilized and will=20
generally be available during the workshop.  =20
=09=A5A syllabus will be provided for workshop participants.=20
 
=09Organizer:  G. J. Nuovo,  SUNY at Stony Brook
 
 
A related symposium will be on=20
 
ENHANCEMENT METHODS FOR IN SITU HYBRIDIZATION=20
 
=09Topic: Fluorescent in situ hybridization (FISH) techniques have=20
been   utilized for the past decade and offer a widely used microscopical=
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tool providing high sensitivity and resolution, as well as the ability to=
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detect multiple targets.  To increase the sensitivity of FISH, indirect=20
amplification by immunological methods has often been the method of=20
choice.  This symposium will include the presentation of several novel=20
approaches using the enzymatic deposition of fluorescent reporter=20
molecules to amplify target localization.  These methods may offer a new=20
approach to  increase both the detection, resolution, and sensitivity of=20
the FISH method.=20
 =09Organizer: S.L. Erlandsen
=09Speakers:=20
=09S. L. Erlandsen,  University of Minnesota, Introduction.
=09A.K. Raap, Leiden University, Sensitive and high resolution FISH=20
=09B. Schmidt, Carnegie Mellon University, Signal amplification in=20
the detection of single copy DNA + RNA by enzyme catalyzed deposition=20
(CARD) of the novel fluorescent reporter substrate, Cy 3.29-tyramide.
=09V.L. Singer, Molecular Probes, Inc.,  The ELF alkaline=20
phosphatase substrate provides a bright, photostable, fluorescent signal=20
amplification method for FISH.=20
 
Another symposium will be on=20
 
APPLICATIONS OF MOLECULAR TECHNOLOGIES TO DIAGNOSTIC AND INVESTIGATIVE=20
PROBLEMS OF BREAST CANCER.=20
 
=09Topic: The symposium will include presentations on  in situ=20
analysis of control of cell proliferation in breast cancer, molecular=20
markers of apoptosis and their correlation with hormone receptor=20
expression and other histologic prognostic markers and the structure of=20
breast tissue as it related to breast cancer development.=20
=09Organizer: A. M. Gown
=09Speakers:=20
=09H. Wolfe, Tufts University,  Molecular markers of apoptosis and=20
their correlation with hormone receptor expression and other histologic=20
prognostic
markers.
=09R. Jensen, Vanderbilt University, BRCA1 gene expression in breast cancer
cells.
=09A.M. Gown, University of Washington, In situ analysis of control=20
of cell proliferation in breast cancer.
=09J.W. Gray, UC San Francisco, Applications of molecular techniques=20
to  diagnostic and investigative problems of breast cancer.
 
 POSTERS ON TOPICS RELATED TO THE WORKSHOP AND SYMPOSIA are welcome =20
(abstract deadline: March 15, 1996) and abstracts  will be published as=20
Proceedings of the Histochemical Society Meeting in the Journal of=20
Histochemistry and Cytochemistry. .
 
CONTACT:=20
Inquiries about program matters and requests for registration materials=20
or abstract forms, and hotel accommodations should be sent to: The=20
Histochemical Society, 4 Barlows Landing Rd., Pocasset, MA 02559=20
(Telephone: 508-563-1155; FAX 508-563-1211 or e-mail: [log in to unmask]). =20
or  inquiries related to the scientific program to:  Dr. W.L. Stahl=20
(e-mail: [log in to unmask]).=20
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