Dear Steve, I assume that you stain two different compartements/organelles
in your red channel. I just can tell you about the smaller network-type
pattern: lt ooks pretty much as a typical ER pattern that appears very
similar in all different kinds of fibroblastic monolayer cells. Although
the "wholes" in the net seem to be rather big for ER, I can tell that
depending on the condition of the cells this happens sometimes  (especially
when the cells were recultured shortly before the fixation).
About the second structure I can just speculate. It looks rather like
cytoskeleton. Would it be possible that you detect a protein that is first
translated at the ER and later bound to some parts of the cytoskeleton?
Hope that helps!
Regards, Rene

>> Does anybody recognize the subcellular region being stained in the
>> enclosed image(red channel). The green channel is a mitotracker stain for
>> mitochondria. This is a 63X image taken on a Zeiss LSM510. While the
>> staining in the red channel seems to partially colocalize with
>> mitochondria (green), it clearly appears different. What might this be?
>> cytoskeletal staining, a different mitochondrion pattern, ER???? If you
>> have some idea please let me know,  much appreciated,  Steve

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Rene C. Rust
Institute of Medical Microbiology
University of Basel
Petersplatz 10
4003 Basel
Switzerland

phone: 0041/61-2673289
fax: 0041/61-2673283
e-mail: [log in to unmask]
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