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hi, i would like one offline copy.Thanks so much.
Xin Zhao [log in to unmask]
----- Original Message ----- 
From: "Jeremy Adler" <[log in to unmask]>
To: <[log in to unmask]>
Sent: Friday, May 18, 2007 5:55 PM
Subject: Re: Colocalisation extended


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Colocalization covers two significantly different conditions ~
(a) that pixels have both fluorophores present
(b) that there is some relationship between the intensities of the 
fluorophores

It is possible to completely meet that first criteria (presence of both 
fluorophores) in the absence any relationship between the inensities.
To reduce the confusion we suggest that mere presence should be called 
Cooccurence and Colocalization be used to refer to any relationship between 
intensities.

A serious problem is all colocalization measurements is the quality of the 
images, imperfect images reduce the measured Pearson colocalization 
coefficient ~  a very common measurement.
A requirement for high quality images appeared in the first quantitative 
colicalilzation paper Manders 1993.   BOLTE and CORDELIÈRES in their review 
In J Microscopy rediscover this problem and then conclude that the Pearson 
corelation coefficient is unusable. A completely over the top conclusion, 
see correspondence in forthcoming issue of J. Microscopy.
The requirement  is for either high quality images or for a method of 
compensating for image noise.
 We have recently introduced a method for making accurate measurements from 
the less than perfect images we actually have. It was presented at the 
Biophysical Soc meeting at Baltimore and at the ELMI meeting in York. 
Contact us offline for copies.


Ingela Parmryd & Jeremy Adler
Cell Biology
The Wenner-Gren Inst.
Arrhenius Laboratories E5
Stockholm University
Stockholm 106 91
Sweden



-----Original Message-----
From: Confocal Microscopy List on behalf of Claire Brown
Sent: Tue 15/05/2007 15:05
To: [log in to unmask]
Subject: Re: Colocalisation extended

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

There was also a recent review of co-localization methods that may be 
helpful.

A guided tour into subcellular colocalization analysis in light microscopy

    * S. BOLTE* *Plateforme d'Imagerie et de Biologie Cellulaire, IFR 87 'la
Plante et son Environnement', Institut des Sciences du Végétal, Avenue de la
Terrasse, 91198 Gif-sur-Yvette Cedex, France &
    * F. P. CORDELIÈRES?

http://www.blackwell-synergy.com/doi/abs/10.1111/j.1365-2818.2006.01706.x