Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Craig
you are correct.
Currently the PARISS only detects X-Y--not depth.
however, most data that I have seen from confocal spectral systems are
only a single XY slice. they usually are not used to do a 3D
reconstruction in spectral mode.
bob
.
Robert M. Zucker, PhD
U.S. Environmental Protection Agency
Office of Research and Development
National Health and Environmental Effects Research Laboratory.
Telephone: 919-541-1585 Fax: 919-541-4017
e-mail: [log in to unmask]
Mail address: Reproductive Toxicology Division, MD 67
2525 E.NC Highway 54
Research Triangle Park, North Carolina, 27711
Shipping address: 2525 E.NC Highway 54
Durham, NC, 27713
Craig Brideau
<craig.brideau@G
MAIL.COM> To
Sent by: [log in to unmask]
Confocal cc
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<CONFOCAL@LISTSE Subject
RV.BUFFALO.EDU> Re: Nikon C1si?
10/05/2007 06:44
PM
Please respond
to
Confocal
Microscopy List
<CONFOCAL@LISTSE
RV.BUFFALO.EDU>
Search the CONFOCAL archive at
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It doesn't seem to deal with depth though? It only detects in X-Y, so
can it generate optical slices?
Craig
On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> The PARISS system uses a Retiga 2000R cooled CCD camera. It is far
more
> sensitive and accurate than any confocal microscope.
> You can detect things in that system that can not be observed with a
> confocal spectral system. It is a great system in my opinion.
> Bob
>
> Robert M. Zucker, PhD
> U.S. Environmental Protection Agency
> Office of Research and Development
> National Health and Environmental Effects Research Laboratory.
> Telephone: 919-541-1585 Fax: 919-541-4017
> e-mail: [log in to unmask]
>
> Mail address: Reproductive Toxicology Division, MD 67
> 2525 E.NC Highway 54
> Research Triangle Park, North Carolina, 27711
>
> Shipping address: 2525 E.NC Highway 54
> Durham, NC, 27713
>
>
>
>
> Craig Brideau
> <craig.brideau@G
> MAIL.COM>
To
> Sent by: [log in to unmask]
> Confocal
cc
> Microscopy List
> <CONFOCAL@LISTSE
Subject
> RV.BUFFALO.EDU> Re: Nikon C1si?
>
>
> 10/05/2007 06:19
> PM
>
>
> Please respond
> to
> Confocal
> Microscopy List
> <CONFOCAL@LISTSE
> RV.BUFFALO.EDU>
>
>
>
>
>
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Will this system have the same sort of sensitivity as a multi-anode
> PMT system like the C1si? This PARISS system seems to use a CCD
> camera as its detector, which will not be as sensitive, if I
> understand correctly?
>
> Craig
>
> On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Criag
> > If spectral analysis is the endpoint you may want to consider the
> PARISS
> > system from Lightform. ( http://www.lightforminc.com/) that can be
> > placed on a widefield microscope.
> > We are using the PARISS system to obtain spectral data --it has 1nm
> > resolution and has a spectrum from 400-900nm. I find it to be a
great
> > asset in the laboratory,
> > It is far more sensitive and accurate that any confocal system that
I
> > have seen.
> >
> > Contact Jeremy Lerner for details on his system.
> > best wishes
> > Bob
> >
> >
> >
> >
> >
> >
> >
> >
> > LightForm, Inc.,
> > 601 Route 206, Suite 26-479
> > Hillsborough NJ 08844
> > Tel: (908)281 9098
> > Email: [log in to unmask]
> >
> >
> >
> >
> >
> >
> > .
> >
> > Robert M. Zucker, PhD
> > U.S. Environmental Protection Agency
> > Office of Research and Development
> > National Health and Environmental Effects Research Laboratory.
> > Telephone: 919-541-1585 Fax: 919-541-4017
> > e-mail: [log in to unmask]
> >
> > Mail address: Reproductive Toxicology Division, MD 67
> > 2525 E.NC Highway 54
> > Research Triangle Park, North Carolina, 27711
> >
> > Shipping address: 2525 E.NC Highway 54
> > Durham, NC, 27713
> >
> >
> >
> >
> > Craig Brideau
> > <craig.brideau@G
> > MAIL.COM>
> To
> > Sent by: [log in to unmask]
> > Confocal
> cc
> > Microscopy List
> > <CONFOCAL@LISTSE
> Subject
> > RV.BUFFALO.EDU> Re: Nikon C1si?
> >
> >
> > 10/05/2007 05:48
> > PM
> >
> >
> > Please respond
> > to
> > Confocal
> > Microscopy List
> > <CONFOCAL@LISTSE
> > RV.BUFFALO.EDU>
> >
> >
> >
> >
> >
> >
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Thanks for your input! As in everything, there's a trade-off.
Still,
> > for us the spectral data will be worth it.
> >
> > Craig
> >
> > On 10/5/07, Robert Zucker <[log in to unmask]> wrote:
> > > Search the CONFOCAL archive at
> > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> > >
> > > Craig
> > > The efficiency of detection is much less in the spectral mode due
to
> > the
> > > design of the multianode detector. In addition as Kurt Thorn said
> the
> > > light is split into a number of different bandpass channels
instead
> of
> > > being detected by only one channel which also limits the detection
> in
> > > each channel.
> > > Nikon compensates for this decreased light by increasing the pixel
> > dwell
> > > time. However photons are photons. With less photons you will get
a
> > > noisier image.
> > > The spectral detection of any confocal system using a multianode
> > > detector will not be as good as a PMT designed for good
sensitivity
> > and
> > > low light detection. It will produce nosier images but if there is
> > > enough light you will be able to determine a valuable spectrum
which
> > can
> > > be used for scientific experiments and to manipulate images. Like
> > > everything with confocal microscopy there is trade-offs and no
> perfect
> > > system.
> > > Best wishes.
> > > Bob
> > >
> > > Robert M. Zucker, PhD
> > > U.S. Environmental Protection Agency
> > > Office of Research and Development
> > > National Health and Environmental Effects Research Laboratory.
> > > Telephone: 919-541-1585 Fax: 919-541-4017
> > > e-mail: [log in to unmask]
> > >
> > > Mail address: Reproductive Toxicology Division, MD 67
> > > 2525 E.NC Highway 54
> > > Research Triangle Park, North Carolina, 27711
> > >
> > > Shipping address: 2525 E.NC Highway 54
> > > Durham, NC, 27713
> > >
> > >
> > >
> > >
> > > Craig Brideau
> > > <craig.brideau@G
> > > MAIL.COM>
> > To
> > > Sent by:
[log in to unmask]
> > > Confocal
> > cc
> > > Microscopy List
> > > <CONFOCAL@LISTSE
> > Subject
> > > RV.BUFFALO.EDU> Re: Nikon C1si?
> > >
> > >
> > > 10/04/2007 02:49
> > > PM
> > >
> > >
> > > Please respond
> > > to
> > > Confocal
> > > Microscopy List
> > > <CONFOCAL@LISTSE
> > > RV.BUFFALO.EDU>
> > >
> > >
> > >
> > >
> > >
> > >
> > > Search the CONFOCAL archive at
> > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> > >
> > > I was wondering if you saw this decreased throughput in spectral
> mode
> > > only, or also in conventional detection mode? The spectral mode
> works
> > > with a multi-anode PMT, which has a lower quantum efficiency than
a
> > > conventional PMT. From what I have seen of the design, it also
has
> > > the option to use a conventional PMT system with the device, and I
> am
> > > wondering if you have also tried that mode of operation?
> > >
> > > Thanks,
> > >
> > > Craig
> > >
> > > On 10/4/07, Robert Zucker <[log in to unmask]> wrote:
> > > > One of the major limitations that we have seen with this unit
and
> > also
> > > > with the Zeiss meta 510 is the decreased light throughput .
This
> > > > creates images that are noisier than conventional confocal
> > > microscopes.
> > > > In our hands it appears you will need to have a bright sample to
> > make
> > > > the spectral system work properly. ,
> > >
> >
>
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