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Hi Carl -

NIS-Elements (Nikon's software) has a tool like this - they call it 
Extended Depth of Focus.  I haven't used it very much but this is 
exactly what it does.

Kurt

Carl Boswell wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dea all,
> Does anyone know of software that selects only in focus portions of an 
> image and discards the out-of-focus parts.  We need to get clear views 
> of a curved surface (the fly eye) with a 10x objective using reflected 
> light.  Only thin optical slices are in focus at this mag, but if we 
> could put together the portions that are in focus over the entire 
> depth of the specimen, we could rebuild a clean image of the 
> structure.  This wouild be a used in an assay screening a bizillion 
> flies, so SEM is not really practical.
> thanks,
> Carl
>
> Carl A. Boswell, Ph.D.
> Molecular and Cellular Biology
> University of Arizona
> 520-954-7053
> FAX 520-621-3709


-- 
Kurt Thorn, PhD
Director, Nikon Imaging Center
University of California San Francisco

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