I have used beads to find the right focal plane.  I put them onto the
coverslip just before I mount them.
Deb Berglund
Montana State University

On Mon, 7 Dec 1998, Bird.Gary wrote:

> Dear All,
>
> Thank you for your responses to my question on focal drift, and I appreciate
> the suggestions for possible causes and cures.
>
> I am equally keen to hear your thoughts on the second part of the question.
> That is, what approach would you suggest whereby the focal position can be
> checked/adjusted to a reference point (not the cells) during an experiment.
> I had pondered if it were possible to use coverslips to which fluorescent
> beads had been attached?  (-how would the beads be attached so that solution
> exchanges would not dislodge them?)
>
> Again, thank you in advance.
>
> Gary Bird