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December 1999

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Subject:
Fluorescence intensity
From:
ROLLAND Agnes <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 23 Dec 1999 14:17:32 +0100
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Hello everyone,

We have a problem using a ZEISS LSM 410 invert confocal microscope with Ar
laser. When we take pictures with FITC labelled proteins at differents
times (60, 90, 120 minutes, etc...), the fluorescence intensity is
increasing with time. It is then impossible to make quantitatives measures
from these pictures.
Do you know this problem?

Cheers,

Agnès ROLLAND

______________________________________

Agnès ROLLAND

INRA - URPOI
Rue de la Géraudière
BP 71627
44316 NANTES CEDEX 03

TEL : 02 40 67 51 44
FAX : 02 40 67 50 66
MAIL : [log in to unmask]

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