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Hi all,

We do 2P microscopy of thick tissue explants. To deliver appropriate oxygen
and carbon dioxide levels to the deep parts of the tissue we bubble the
medium with 95% O2 and 5% CO2. That keeps the cells as happy as when they
are vascularized.

Now we want to do the experiments with serum added to the medium, but the
extra protein causes the bubbling medium to foam too much.

Any suggestions on how to increase the dissolved O2 and CO2 without the
foaming?
Also, any ideas on how to monitor the resulting O2 levels?

Thanks so much.


Paul Herzmark

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Department of Molecular and Cell Biology
479 Life Science Addition
University of California, Berkeley
Berkeley, CA  94720-3200
(510) 643-9603