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I've looked at Qdots using 2P, 1P, and widefield and they have always
been pretty bright. Can the "blinking" phenomena be an issue? Do they
even still do that?
Best,
Gary Laevsky, Ph.D.
Imaging Application Specialist
Andor Technology
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Eric Scarfone
Sent: Thursday, August 30, 2007 11:14 AM
To: [log in to unmask]
Subject: Re: Single Q-dots for PSF measurement
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
hello
if I remember well, Q-dots are remarquably bright using multiphoton
excitation (check out the original Zipfel paper). In my hands, using
continuous light sources, they are bright but not that much. I never
tried to dilute them that much that I could visualise subresolution
dots.
Eric
Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet
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CFH, M1:02
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SE-171 76 Stockholm, Sweden
Work: +46 (0)8-517 70343,
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----- Original Message -----
From: Lauran Oomen <[log in to unmask]>
Date: Thursday, August 30, 2007 11:35 am
Subject: Single Q-dots for PSF measurement
To: [log in to unmask]
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Hello all,
>
> We have tried to use Q-dots for PSF measurements on our confocals,
> but in our hands they are not bright enough and also bleach. I
> guess a number of you have tried Q-Dots as well, since they promise
> to be good sub-resolution targets. I have searched the archive and
> only found a message about this dating back to 2004, also reporting
> negative results. Did anyone successfully use Q-dots as sub-
> resolution targets to measure the PSF of a confocal? If yes, could
> you share your protocol with us?
>
> Lauran
> *************************************
> Lauran Oomen
> The Netherlands Cancer Institute
> Digital Microscopy Facility (H0)
> PO box 90203
> 1006BE Amsterdam
> The Netherlands
> tel: +31 20 5126080
> fax: +31 20 5122909
>
>
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