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Another issue, in addition to the tryptophan fluorescence noted by Mike, is
the excitation light's transmittance through the objective.  Many
objectives have little to no transmittance of light below 300nm.

Ryan Schreiner

On Tue, Jan 24, 2017 at 10:08 AM Darren Thomson <
[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello All,
>
> We have a compound which annoyingly excites at 280nm. We need to detect
> whether it has entered the cell or not.
> Is anyone aware of an excitation source this deep? Would we need to go
> back to mercury lamps and try tease out with a custom filter?
>
> Cheers,
>
> Dr Darren Thomson
> Manchester Fungal Infection Group, UK.
>