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March 2001

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Subject:
Re: immunofluorescence MetOH GFP
From:
Ute Frevert <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 7 Mar 2001 11:13:33 -0500
Content-Type:
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We are working with malaria parasites expressing GFP in their
cytoplasm. Frustratingly, the GFP signal is greatly diminished if not
abolished after paraformaldehyde fixation. Instead, we see a marked
increase in autofluorescence. I wonder if others had similar
experiences.

Ute


>Dear confocalist,
>
>I'm trying to make immunofluorescence of Tubulin in GFP transfected
>cells. I have a good staining fixing with cold methanol but I loose the
>GFP signal. With PF the tubulin staining is not so good. Any suggestion?
>
>Susanna
--
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Ute Frevert, D.V.M., Ph.D.
New York University School of Medicine
Department of Medical and Molecular Parasitology
341 E 25 St
New York, NY 10010
Tel:   212-263-6755
FAX: 212-263-8116
e-mail: [log in to unmask]

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